A7607
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 | Immunofluorescence Mouse colon sections, paraffin embedded-ethanol fixed were stained with 10 μg/mL of Monoclonal Anti-Actin, smooth Muscle Specific (Cat. No. A7607). The antibody was developed with Goat Anti-Mouse IgG, FITC conjugate (Cat. No. F8771). |
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A7607
Sigma
Monoclonal Anti-Actin, Smooth Muscle antibody produced in mouse
~1.0 mg/mL, clone CGA7, purified immunoglobulin, buffered aqueous solution
Description
| Immunogen | partially purified chicken gizzard actin.1 |
| Specificity | The antibody specifically recognizes α and γ smooth muscle actin. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | CGA7 (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~42 kDa |
| species reactivity | monkey, chicken, human, rat, mouse |
| concentration | ~1.0 mg/mL |
| application(s) | immunoblotting: 5-10 μg/mL using chicken gizzard total extract |
| immunocytochemistry: suitable | |
| immunohistochemistry1,2: suitable | |
| indirect ELISA: suitable | |
| isotype | IgG2b |
| Gene Information | human ... ACTA2(59) |
| rat ... Acta2(81633) | |
| mouse ... Acta2(11475) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| Cited Reference | 1. Gown, A.M., et al. J. Cell Biol. 100, 807-813, (1985) Abstract |
| 2. Charbord, P., et al. Blood 5, 1138-1142, (1985) |
